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Related Experiment Video

Updated: May 28, 2026

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
07:09

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

Published on: July 31, 2012

Tools for coproducing multiple proteins in mammalian cells.

Zahra Assur1, Wayne A Hendrickson, Filippo Mancia

  • 1Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 12, 2011
PubMed
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Researchers developed an efficient fluorescence marking method to create stable mammalian cell lines overexpressing multiple proteins. This technique improves the production of complex recombinant proteins like antibody fragments, overcoming common expression challenges.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Background:

  • Mammalian cell systems are crucial for producing functional recombinant multiprotein complexes.
  • Challenges include low expression yields, lengthy generation times for cell lines, and high costs.
  • Coexpressing multiple proteins requires complex screening and selection processes.

Purpose of the Study:

  • To present an efficient fluorescence marking procedure for establishing stable mammalian cell lines overexpressing multiple proteins coordinately.
  • To validate the method for producing recombinant monoclonal antibody Fab fragments.
  • To offer a scalable solution for more complex multi-component expression systems.

Main Methods:

  • Development of a novel fluorescence marking technique.

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Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
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Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
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Published on: June 27, 2017

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Last Updated: May 28, 2026

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
07:09

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

Published on: July 31, 2012

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
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Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

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  • Establishment of stable mammalian cell lines engineered for coordinated protein overexpression.
  • Validation using recombinant monoclonal antibody Fab fragment production.

    • Main Results:

    • Successful establishment of stable cell lines overexpressing two proteins in coordination.
    • Efficient production of recombinant monoclonal antibody Fab fragments validated the method's efficacy.
    • Demonstrated potential for expansion to systems with more than two protein components.

    Conclusions:

    • The fluorescence marking procedure offers an efficient solution for producing recombinant multiprotein complexes in mammalian cells.
    • This method addresses key challenges in expression yield, time, and cost.
    • The technique is adaptable for complex systems, facilitating advanced research in structural and functional studies.