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Updated: May 28, 2026

Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction
Published on: July 26, 2022
SEM observation of wet biological specimens pretreated with room-temperature ionic liquid
Tetsuya Tsuda1, Noriko Nemoto, Koshi Kawakami
1Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan. ttsuda@chem.eng.osaka-u.ac.jp
Abstract:
A facile pretreatment process for SEM: The use of room temperature ionic liquids (RTILs) provides an interesting method for SEM of biological specimens. We used a novel and concise method of pretreatment, excluding fixation or Au sputtering steps. Fine and smooth-textured SEM images of a wide variety of biological specimens treated in this way were observed without artefacts.
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Preparation of Samples for Electron Microscopy
Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

