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Related Experiment Video

Updated: May 28, 2026

Implementation of a Reference Interferometer for Nanodetection
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Implementation of a Reference Interferometer for Nanodetection

Published on: April 26, 2014

Determination of binding kinetics using whispering gallery mode microcavities.

Carol E Soteropulos, Heather K Hunt, Andrea M Armani

    Applied Physics Letters
    |October 13, 2011
    PubMed
    Summary

    This study introduces a novel silica microsphere resonator platform for analyzing biomolecular interactions. The biosensor effectively measures binding kinetics, demonstrating potential for determining dissociation constants in real-time.

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    Area of Science:

    • Biophotonics
    • Biosensing
    • Biomolecular Interactions

    Background:

    • Optical microcavity sensors offer sensitive detection of biomolecular binding.
    • Understanding binding kinetics is crucial for characterizing molecular interactions.
    • Silica microsphere resonators are promising platforms for high-sensitivity sensing.

    Purpose of the Study:

    • To develop and demonstrate a novel bioconjugated silica microsphere resonator platform.
    • To investigate the binding kinetics of the biotin-streptavidin system using this platform.
    • To evaluate the potential of whispering gallery mode resonators for kinetic analysis.

    Main Methods:

    • Fabrication and characterization of bioconjugated silica microsphere resonators.
    • Verification of covalent biotin attachment to the resonator surface.

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    Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
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    Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

    Published on: May 1, 2012

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    Last Updated: May 28, 2026

    Implementation of a Reference Interferometer for Nanodetection
    16:11

    Implementation of a Reference Interferometer for Nanodetection

    Published on: April 26, 2014

    Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
    09:35

    Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

    Published on: May 1, 2012

  • Real-time detection experiments for streptavidin binding.
  • Preliminary kinetic analysis of binding data.

    • Main Results:

    • The developed platform exhibited stable optical performance.
    • Covalent attachment of biotin was successfully confirmed.
    • Streptavidin binding was detected with high sensitivity.
    • Preliminary kinetic analysis yielded a dissociation constant in agreement with literature values.

    Conclusions:

    • Bioconjugated silica microsphere resonators provide a viable platform for studying biomolecular binding kinetics.
    • Whispering gallery mode resonators show significant potential for accurate determination of binding constants.
    • This technology can advance the understanding of fundamental biomolecular interactions.