Related Experiment Video
Updated: May 28, 2026
Environmental Screening of Aeromonas hydrophila, Mycobacterium spp., and Pseudocapillaria tomentosa in Zebrafish Systems
Published on: December 8, 2017
[Screening of high-yield PUFAs Mortierella isabellina strain]
Ben-Bo Xu1, Min Ba, Ling-Li Xie
1Yangtze University, Jingzhou, China. benboxu@yangtzeu.edu.cn
Abstract:
The original strain Mortierella isabellina As3.3410 was treated by microwave and ultraviolet. Mutated strains were screened by acetyl salicylic acid and low temperature (15°C). A high-yield strain named as A35-4 was successfully selected. The biomass of this strain was 17.9 g/L, oil content was 67.8%, oil production was 12.12 g/L, polyunsaturated fatty acids (PUFAs) content was 20.2%, and production of PUFAs was 2.46 g/L, which increased 32.6%, 49.8%, 98.69%, 14.0%, and 125.7% compared with the original A0 stain, respectively. The continuous slope transmission experiments confirmed that the strain had a good genetic stability. The study is beneficial for cloning high efficiency genes for PUFAs and producing PUFAs in this stain, and lays the ground work for creation of transgenic plants containing high levels of PUFAs.
Insights
Researchers developed a high-yield fungal strain, A35-4, for enhanced production of polyunsaturated fatty acids (PUFAs). This strain significantly boosts biomass, oil, and PUFA yields, offering a stable source for valuable compounds.
Area of Science:
- Microbial biotechnology
- Fungal strain development
- Bioprocess engineering
Context:
- Mortierella isabellina is a fungus known for its potential in producing valuable lipids.
- Optimizing fungal strains for industrial applications requires targeted mutation and screening strategies.
- Polyunsaturated fatty acids (PUFAs) are essential nutrients with significant health benefits and commercial value.
Purpose:
- To improve the yield of polyunsaturated fatty acids (PUFAs) from Mortierella isabellina.
- To develop a high-performing fungal strain through mutagenesis and selective screening.
- To assess the genetic stability of the selected high-yield strain for reliable production.
Summary:
- The original Mortierella isabellina As3.3410 strain was subjected to microwave and ultraviolet treatments.
- Mutated strains were screened using acetyl salicylic acid and low temperatures (15°C), leading to the selection of a high-yield strain, A35-4.
- Strain A35-4 demonstrated significantly increased biomass (17.9 g/L), oil content (67.8%), oil production (12.12 g/L), PUFA content (20.2%), and PUFA production (2.46 g/L) compared to the original strain.
- Continuous transmission experiments confirmed the good genetic stability of the A35-4 strain.
Impact:
- The study provides a robust platform for enhancing polyunsaturated fatty acid (PUFA) production in fungi.
- It facilitates the cloning of high-efficiency genes for PUFA biosynthesis.
- Lays the groundwork for developing transgenic plants with elevated PUFA levels.
