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In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients
Published on: August 12, 2017
Lymphocyte subtypes CD3+, CD19+, CD16+CD56+, CD4+, CD8+, and CD3+HLA-DR+ in peripheral blood obtained from patients
S Zegleń1, A Łaszewska, J Wojarski
1Department and Clinic of Cardiac Surgery and Transplantology, Silesian Centre for Heart Diseases, Zabrze, Poland. slawekzeglen@poczta.onet.pl
Insights
Peripheral blood lymphocyte counts, including CD19+ B cells and CD8+ T cells, significantly decreased one year after thoracic organ transplantation. Numbers trended toward normalization after the first year, with increased activated HLA-DR+ cells observed.
Area of Science:
- Immunology
- Transplantation Medicine
- Clinical Research
Background:
- Assessing peripheral blood lymphocyte subtypes in thoracic organ recipients is crucial for understanding post-transplant immune status.
- Specific lymphocyte populations evaluated include CD3+, CD19+, CD16+CD56+, CD4+, CD8+, and CD3+HLA-DR+.
Purpose of the Study:
- To evaluate changes in peripheral blood lymphocyte subtypes at various time points after lung and heart transplantation.
- To identify trends in lymphocyte populations during the first year and beyond post-transplantation.
Main Methods:
- Seventeen lung transplant (LT) and 5 heart transplant (HT) recipients were studied.
- Lymphocyte phenotypes were analyzed using the Simultest IMK Plus assay.
- Data collected at various intervals post-transplantation.
Main Results:
- A significant decrease in CD19+ B lymphocytes (56%), CD8+ T cells (48%), and CD16+CD56+ natural killer cells (56%) was observed one year post-transplantation.
- Conversely, an increase in activated lymphocytes (CD3+HLA-DR+) was noted.
- A trend towards normalization of lymphocyte parameters was observed after the first year.
Conclusions:
- Thoracic organ recipients exhibit a decrease in various peripheral blood lymphocyte subtypes within the first year post-transplantation, excluding activated HLA-DR+ cells.
- A slow restoration of lymphocyte populations occurs after the first year.
- These findings highlight dynamic immune changes following thoracic organ transplantation.
Introduction:
The aim of this study was to assess peripheral blood lymphocyte subtypes (CD3+, CD19+, CD16+CD56+, CD4+, CD8+, and CD3+HLA-DR+) obtained from thoracic organ recipients at various periods after transplantation.
Material And Methods:
Seventeen patients after lung transplantation (LT) and 5 patients after heart transplantation (HT) included 13 males (76.5%) and 4 females (23.5%) of overall mean age at the time of transplantation of 46.7±11.55 years and mean body mass index of 21.1±4. Lymphocyte phenotypes were estimated using Simultest IMK Plus.
Results:
A significant decrease in lymphocytes of the majority of subtypes was observed at 1 year posttransplantation compared with normal ranges: CD19+ B lymphocytes in 56% of patients, CD8+ T cells among 48% and CD16+CD56+ natural killer elements, 56%. In contrast, there were increased numbers of activated lymphocytes (CD3+HLA-DR+). Beyond the 1-year observation, we observed a trend to normalize parameters among the majority of subjects.
Conclusion:
A clear tendency to a decrease number of peripheral blood lymphocytes of various subtypes was observed among thoracic organ recipients in the first year posttransplantation with the exception of activated HLA-DR+ cells. After the first year, there was slow restoration of lymphocytes.
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