Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genetic analysis of clathrin function in yeast.

G S Payne1

  • 1Department of Biological Chemistry, University of California, Los Angeles School of Medicine 90024.

The Journal of Membrane Biology
|June 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Environmental & load data: 1:15 Scale tidal turbine subject to a variety of regular wave conditions.

Data in brief·2019
Same author

Validating a robust double-quantum-filtered (1) H MRS lactate measurement method in high-grade brain tumours.

NMR in biomedicine·2016
Same author

Multivariate modelling of prostate cancer combining magnetic resonance derived T2, diffusion, dynamic contrast-enhanced and spectroscopic parameters.

European radiology·2015
Same author

Evaluation of lactate detection using selective multiple quantum coherence in phantoms and brain tumours.

NMR in biomedicine·2015
Same author

Functional MRI and CT biomarkers in oncology.

European journal of nuclear medicine and molecular imaging·2015
Same author

Effect on therapeutic ratio of planning a boosted radiotherapy dose to the dominant intraprostatic tumour lesion within the prostate based on multifunctional MR parameters.

The British journal of radiology·2014

Yeast studies reveal clathrin (a protein coat) is not essential for all secretory pathway transport but is crucial for retaining specific Golgi proteins. This challenges previous models and supports a broader role in sorting cargo molecules.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Clathrin-coated membranes are vital for intracellular transport.
  • Yeast mutants offer a model to study clathrin function in vivo.
  • Previous research proposed clathrin's essential role in the secretory pathway.

Purpose of the Study:

  • To investigate the function and dynamics of clathrin in yeast.
  • To clarify clathrin's role in the secretory pathway and protein retention.
  • To reconcile findings in yeast with clathrin's proposed functions in mammalian cells.

Main Methods:

  • Phenotypic characterization of clathrin-deficient yeast mutants.
  • Genetic analysis of yeast strains with disrupted clathrin genes.
  • Biochemical experiments to study protein retention mechanisms.

Related Experiment Videos

Main Results:

  • Clathrin is not essential for vesicle formation at the ER or Golgi.
  • Clathrin is required for the intracellular retention of the Golgi protein Kex2p.
  • Clathrin-deficient yeast exhibit normal protein secretion and endocytosis.

Conclusions:

  • Clathrin's role in the secretory pathway is more nuanced than previously thought.
  • Clathrin acts as a facilitator in intracellular transport by sorting and concentrating cargo.
  • Further research is needed to unify clathrin's function across different eukaryotic organisms.