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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
Published on: March 17, 2023
Multiplex spectral surface plasmon resonance imaging (SPRI) sensor based on the polarization control scheme.
Chi Lok Wong1, George Chung Kit Chen, Beng Koon Ng
1Division of Microelectronics, School of Electrical & Electronic Engineering, Nanyang Technological University, 50 Nanyang Drive, Singapore 639798, Singapore. wongchilok@ntu.edu.sg
This study introduces a novel 2D spectral Surface Plasmon Resonance (SPR) sensor with enhanced resolution and multiplexing capabilities. The advanced sensor achieves significant improvements in detecting bio-molecular interactions for applications in proteomics and drug discovery.
Area of Science:
- * Photonics and Plasmonics
- * Biosensing and Bio-imaging
- * Nanotechnology
Background:
- * Surface Plasmon Resonance (SPR) is a label-free optical technique widely used for detecting molecular interactions.
- * Existing 2D spectral SPR sensors face limitations in resolution and multiplexing capacity for high-throughput biological analysis.
Purpose of the Study:
- * To develop and demonstrate a novel two-dimensional (2D) spectral SPR sensor utilizing a polarization control scheme.
- * To significantly enhance sensor resolution and multiplexing capabilities for advanced bio-molecular detection.
Main Methods:
- * Implementation of a polarization control configuration to convert SPR-induced phase differences into spectral color responses.
- * Fabrication and testing of an 8x4 sensor array for simultaneous monitoring of 32 samples.
- * Demonstration of antigen-antibody binding detection using Bovine Serum Albumin (BSA).
Main Results:
- * Achieved a sensor resolution of 2.7 x 10^-6 RIU, representing a 26-fold improvement over existing 2D spectral SPR sensors.
- * Successfully demonstrated 2D multiplex array detection, monitoring 32 samples simultaneously.
- * Established a detection limit of 21 ng/ml for BSA detection, 36 times lower than previously reported phase imaging SPR sensors.
Conclusions:
- * The developed 2D spectral SPR imaging sensor offers high sensitivity, 2D multiplex detection, and real-time response.
- * This technology holds significant promise for rapid, high-throughput, label-free, and multiplexed protein array detection.
- * Potential applications include proteomics, biomarker screening, disease prognosis, and drug discovery.
