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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Updated: May 28, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
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Localization-based super-resolution microscopy with an sCMOS camera.

Zhen-Li Huang1, Hongyu Zhu, Fan Long

  • 1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China. sqzeng@mail.hust.edu.cn

Optics Express
|October 15, 2011
PubMed
Summary
This summary is machine-generated.

Scientific complementary metal-oxide-semiconductor (sCMOS) cameras can now be used for localization microscopy. These cameras, when paired with bright probes, achieve imaging in the shot-noise-limited region.

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Area of Science:

  • Optical Microscopy
  • Biophysics
  • Nanotechnology

Background:

  • Localization microscopy traditionally requires electron multiplying charge coupled device (EMCCD) cameras due to presumed weak single-molecule emission.
  • The necessity of EMCCDs for eliminating read noise in super-resolution imaging is a long-standing assumption.

Purpose of the Study:

  • To evaluate the feasibility of using scientific complementary metal-oxide-semiconductor (sCMOS) cameras for localization microscopy.
  • To assess the noise performance and imaging capabilities of sCMOS cameras in this application.

Main Methods:

  • Experimental demonstration of sCMOS camera performance in localization microscopy.
  • Characterization of sCMOS camera noise performance using a bright fluorescence probe (d2EosFP).

Main Results:

  • sCMOS cameras successfully imaged actin bundles with a Full Width at Half Maximum (FWHM) diameter of 37 nm.
  • Localization microscopy using sCMOS cameras and d2EosFP operates within the shot-noise-limited regime.

Conclusions:

  • sCMOS cameras are a viable alternative detector for localization microscopy.
  • The use of bright probes enables sCMOS cameras to achieve high-resolution imaging, challenging previous assumptions about detector requirements.