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Updated: May 28, 2026

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
Published on: December 1, 2018
Low optical power reference detector implemented in the validation of two independent techniques for calibrating
Jessica Y Cheung1, Christopher J Chunnilall, Geiland Porrovecchio
1National Physical Laboratory, Teddington TW11 0LW, UK. jessica.cheung@npl.co.uk
Abstract:
We introduce a technique for measuring detection efficiency that is traceable to the primary standard, the cryogenic radiometer, through a reference silicon photodiode trap detector. The trap detector, used in conjunction with a switched integrator amplifier, can measure signals down to the 0.1 pW (3 x 10⁵ photons second-1) level with 0.1% uncertainty in a total integration time of 300 seconds. This provides a convenient calibration standard for measurements at these levels across the optical spectrum (UV - near IR). A second technique is also described, based on correlated photons produced via parametric down-conversion. This can be used to directly measure detection efficiency in the photon counting regime, and provides a route for expanding the formulation of the candela in terms of photon flux to enable it to address the needs of emerging quantum optical technologies and applications. The two independent techniques were cross-validated by a comparison carried out at 702.2 nm, which showed agreement to within 0.2%.
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