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Updated: May 28, 2026

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
Published on: November 28, 2019
Human amniotic glycodelin actively regulates changes in β-catenin immunoreactivity in cultured human umbilical vein
Alessandro Pala1, Piera D'Elia, Giuseppe Spampinato
1Department of Gynecological-Obstetric Sciences and Urological Sciences, Sapienza University of Rome, Rome, Italy. alessandro.pala@uniroma1.it
Objective:
Assessment of the capacity of Glycodelin A (GdA) to modulate the aggregation of cultured human umbilical vein endothelial cells.
Methods:
Highly purified Glycodelin A (GdA) from late first trimester amniotic fluid has been added to cultured cells and its biological activity has been observed with immunofluorescent staining of β-catenin molecules.
Results:
GdA induces translocation of β-catenin molecules promoting cell-to-cell adhesion and formation of adherents junctions through cytoskeletal reorganization.
Conclusion:
These data provide further mechanistic insight into the specificity of cell-to-cell adhesion, thus corroborating the role of GdA in promoting angiogenesis.
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