Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Architecture and function of holocentric CENP-A-independent inner kinetochores.

Science advances·2026
Same author

SIRPα ablated iPSC-derived macrophages resist hypophagia and enhance mAb-dependent and CAR-mediated cytotoxicity of solid tumors.

Molecular therapy. Oncology·2026
Same author

SEMPLR: an R package for transcription factor binding prediction.

Bioinformatics (Oxford, England)·2026
Same author

CRISPR-mediated engineering of bovine satellite cells for Alpha-Gal Syndrome-compatible cultivated meat.

bioRxiv : the preprint server for biology·2026
Same author

Direct RNA Sequencing reveals epitranscriptomic regulation of brain cells and Alzheimer's Disease pathology.

bioRxiv : the preprint server for biology·2026
Same author

Antibody-Mediated Delivery of BRM/BRG1 Protein Degraders Affords Strong Antitumor Efficacy in Multiple BRM-Dependent Non-Small Cell Lung Cancer Xenograft Models.

Journal of medicinal chemistry·2026
Same journal

Conformational mobility of cationic voltage-gated ion channels.

Critical reviews in biochemistry and molecular biology·2026
Same journal

DNA polymerase I: structure, activity, and function in bacterial DNA replication and repair.

Critical reviews in biochemistry and molecular biology·2026
Same journal

The impact of HIV-1 Nef on CD4<sup>+</sup> T cells.

Critical reviews in biochemistry and molecular biology·2026
Same journal

Recent insights into bacterial ESCRT-III-mediated membrane remodeling.

Critical reviews in biochemistry and molecular biology·2026
Same journal

CRISPR-Cas9 editing of agricultural crops and medicinal plants: toward a cornucopia of natural products.

Critical reviews in biochemistry and molecular biology·2025
Same journal

RNA modifications as a means of self-recognition and immune protection.

Critical reviews in biochemistry and molecular biology·2025
See all related articles

Related Experiment Video

Updated: May 28, 2026

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
08:56

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

Proteomics and pluripotency.

Justin Brumbaugh1, Christopher M Rose, Douglas H Phanstiel

  • 1Department of Biomolecular Chemistry, University of Wisconsin-Madison, USA.

Critical Reviews in Biochemistry and Molecular Biology
|October 18, 2011
PubMed
Summary
This summary is machine-generated.

Mass spectrometry (MS) and stem cell biology integration offers novel insights into cellular processes. This synergy enables large-scale analysis of post-transcriptional regulation crucial for pluripotency and differentiation.

More Related Videos

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
07:18

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

Related Experiment Videos

Last Updated: May 28, 2026

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
08:56

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
07:18

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

Area of Science:

  • Proteomics
  • Stem Cell Biology
  • Mass Spectrometry

Background:

  • Mass spectrometry (MS) and stem cell biology have independently seen significant advancements.
  • The intersection of these fields offers unique insights into biological mechanisms and technological capabilities.

Purpose of the Study:

  • To highlight the synergistic relationship between MS and stem cell biology.
  • To emphasize how MS-based proteomics can investigate fundamental aspects of pluripotency and differentiation.

Main Methods:

  • Utilizing mass spectrometry-based proteomics to analyze biological samples.
  • Applying targeted and high-throughput proteomic approaches.

Main Results:

  • MS-based proteomics allows tracking of post-transcriptional regulation and signaling pathways.
  • These pathways are critical for stem cell pluripotency and differentiation.
  • Enables large-scale examination of biological processes difficult to study otherwise.

Conclusions:

  • The integration of MS and stem cell biology provides powerful tools for biological discovery.
  • This interdisciplinary approach is essential for dissecting complex cellular mechanisms like pluripotency and differentiation.