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Related Experiment Video

Updated: May 28, 2026

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

Published on: July 30, 2011

Gene expression profiling of in Moniezia expansa at different developmental proglottids using cDNA microarray.

Xinwen Bo¹, Wenjuan Zhao, Hui Zhang

¹Genetic Engineering Laboratory, The Breed & Biotechnology Key Laboratory of Sheep in XinJiang Bingtuan/Animal Husbandry and Veterinary Institute, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, 832000, People's Republic of China. boxinwen@yahoo.com.cn

Molecular Biology Reports

|October 18, 2011

Summary

Gene expression analysis reveals significant changes in Moniezia expansa proglottids during development. This study identifies key genes involved in tapeworm pathogenesis and reproductive organ development.

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Area of Science:

Parasitology
Molecular Biology
Genomics

Background:

Moniezia expansa is an economically significant tapeworm parasite.
Understanding tapeworm gene expression is crucial for developing control strategies.

Purpose of the Study:

To profile gene expression in Moniezia expansa proglottids at different developmental stages.
To identify genes involved in tapeworm development and pathogenesis.

Main Methods:

cDNA microarray analysis of 4,056 gene spots from immature, mature, and gravid proglottids.
Real-time quantitative polymerase chain reaction (RT-PCR) for expression validation.
Data deposited in NCBI Gene Expression Omnibus (GEO Series accession number GSE13982).

Main Results:

Significant up-regulation and down-regulation of genes observed across developmental stages compared to scolex-neck controls.
Identified genes include transcription factors involved in metabolism, protein biosynthesis, cell differentiation, and nucleic acid binding.
Expression levels of specific genes (UBE2A, Cavβ, RAD51, DAZ, PKAc) were confirmed by RT-PCR.

Conclusions:

Gene expression profiles provide insights into Moniezia expansa development and pathogenesis.
Identified genes are critical for understanding tapeworm reproductive organ development and potential drug targets.