Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Two new dactylogyrid species (Platyhelminthes: <i>Monogenea</i>) parasitising the gills of <i>Garra gotyla</i> (Cyprinidae) in India: morphological and molecular characterization.

Journal of helminthology·2025

Same author

Phase evolution and morphological transformation of high-entropy alloy FeMnNiAlSiC nanoparticles <i>via</i> sequential picosecond laser ablation and nanosecond laser annealing.

RSC advances·2025

Same author

Ten-year survival rates by PSA nadir in patients with metastatic hormone-sensitive prostate cancer: long-term survival analysis from the ECOG-ACRIN 3805 (CHAARTED) trial.

Annals of oncology : official journal of the European Society for Medical Oncology·2025

Same author

Multicenter analysis of high-dose chemotherapy regimens for the treatment of patients with refractory or recurrent germ cell tumors.

ESMO open·2025

Same author

Evaluating common bean elite lines in two contrasting ecologies of north India: eco-phenological dynamics and environment relations to crop yields.

International journal of biometeorology·2025

Same author

Development of simplex and quintuplex RT-PCR for simultaneous detection of soybean viruses.

Journal of virological methods·2024

Same journal

A 3D-printed microdevice for dielectrophoretic torque-driven rotation of dielectric microspheres to support development of manipulation systems for zebrafish eggs.

Journal of micromechanics and microengineering : structures, devices, and systems·2026

Same journal

Polymeric microbead arrays for microfluidic applications.

Journal of micromechanics and microengineering : structures, devices, and systems·2025

Same journal

On-ratio PDMS bonding for multilayer microfluidic device fabrication.

Journal of micromechanics and microengineering : structures, devices, and systems·2024

Same journal

The UmboMic: A PVDF Cantilever Microphone.

Journal of micromechanics and microengineering : structures, devices, and systems·2024

Same journal

Heterogeneous micro-architectonic integration of SU-8 and highly entangled polyacrylamide hydrogel to realize cut-resistant soft superhydrophobic surfaces.

Journal of micromechanics and microengineering : structures, devices, and systems·2024

Same journal

Effects of Micromachining on Anti-oxidant Elution from a Mechanically-Adaptive Polymer.

Journal of micromechanics and microengineering : structures, devices, and systems·2024

See all related articles

Search research articles

Related Experiment Video

Updated: May 28, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

A doublet microlens array for imaging micron-sized objects.

A Tripathi¹, N Chronis

¹Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA.

Journal of Micromechanics and Microengineering : Structures, Devices, and Systems

|October 18, 2011

Summary

This summary is machine-generated.

Researchers developed a novel doublet microlens array for imaging tiny objects. This miniaturized optical module offers a cost-effective solution for advanced imaging and lab-on-chip applications.

More Related Videos

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

Related Experiment Videos

Last Updated: May 28, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

Area of Science:

Optics and Photonics
Microfabrication
Nanotechnology

Background:

Micron-sized object imaging requires advanced optical solutions.
Existing miniaturized optical modules are often expensive and lack integration capabilities.

Purpose of the Study:

To develop a high-numerical aperture, doublet microlens array for imaging micron-sized objects.
To create an inexpensive and miniaturized optical module for integration with microfluidic and photonic devices.

Main Methods:

Fabrication of a silicon microhole array using standard microfabrication processes.
Novel fluidic assembly of glass microspheres on the microhole array.
Covering the microspheres with a thin polymer layer to form doublet microlenses.
Achieved an array of 56 micrometer diameter microlenses with a numerical aperture of approximately 0.5.

Main Results:

Demonstrated brightfield and fluorescent image formation of micron-sized objects.
Direct imaging onto a CCD sensor without intermediate lenses.
Successful integration of microlenses with microfluidic devices is feasible.

Conclusions:

The proposed doublet microlens array technology is a significant advancement for miniaturized optical modules.
Offers a cost-effective solution for high-end imaging and detection applications.
Enables integration with lab-on-chip and photonic chips for diverse applications.