Related Experiment Video
Updated: May 28, 2026

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
Published on: May 27, 2022
Molecular identification tools for sibling species of Scedosporium and Pseudallescheria
M Lackner1, C H Klaassen, J F Meis
1Federal Institute for Drugs and Medical Devices, Bonn, Germany.
Abstract:
The aim of this study was to develop molecular identification tools for currently recognized species of Pseudallescheria and Scedosporium through the use of species-specific primers and RFLP, so as to enhance rapid differentiation of clinically relevant species. The variability of species was established in a set of 681 Internal Transcribed Spacer (ITS) and 349 ß-tubulin (BT2) sequences. Amplified Fragment Length Polymorphism profile clustering matched with BT2 results, whereas ITS grouping was less detailed. ITS was sufficient for the differentiation of most haplotypes of clinically relevant species (P. apiosperma, P. boydii, S. aurantiacum, S. dehoogii, and S. prolificans) and of environmental species (P. minutispora and Lophotrichus fimeti) when Restriction Fragment Length Polymorphism (RFLP) were applied. For the identification of P. apiosperma and P. boydii species-specific BT2 primers were needed. Pseudallescheria fusoidea, P. ellipsoidea and P. angusta remained difficult to distinguish from P. boydii.
Related Concept Videos
Methods of Classification and Identification
Modern Molecular Taxonomy
Phylogenetic Species Concept in Microbiology
Rapid Identification of Pathogens
Applications of Molecular Taxonomy

