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Quantitative aspects of septicemia.

P Yagupsky1, F S Nolte

  • 1Department of Microbiology and Immunology, University of Rochester Medical Center, New York 14642.

Clinical Microbiology Reviews
|July 1, 1990
PubMed
Summary
This summary is machine-generated.

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Quantitative blood cultures, once limited, are now widely used thanks to lysis-centrifugation methods. These techniques improve sepsis diagnosis and management by accurately quantifying bacteria in blood samples.

Area of Science:

  • Medical Microbiology
  • Infectious Diseases
  • Clinical Diagnostics

Background:

  • Traditional quantitative blood cultures were impractical for diagnosing sepsis due to cumbersome methods and small blood volumes.
  • Lysis-centrifugation methods have overcome previous limitations, enhancing the utility of quantitative blood cultures.

Purpose of the Study:

  • To highlight the evolution and improved utility of quantitative blood cultures in clinical microbiology.
  • To discuss the applications of quantitative blood cultures in diagnosing and managing various infections.

Main Methods:

  • Development and commercial availability of lysis-centrifugation direct plating methods for blood cultures.
  • Comparison of lysis-centrifugation methods with traditional broth-based blood culture techniques.

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Main Results:

  • Lysis-centrifugation methods demonstrate good performance, making quantitative blood cultures widely adopted.
  • Bacteremia magnitude varies with patient age, correlating with disease severity and mortality in certain populations.
  • Quantitative cultures aid in diagnosing catheter-related sepsis and differentiating contamination from true bacteremia.

Conclusions:

  • Quantitative blood cultures, particularly with lysis-centrifugation, are now essential tools in clinical microbiology.
  • These methods offer valuable insights into sepsis, endocarditis, and catheter-related infections.
  • While powerful, quantitative criteria alone cannot always distinguish contamination from true bacteremia.