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Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana
Published on: May 21, 2019
Apoplastic reactive oxygen species transiently decrease auxin signaling and cause stress-induced morphogenic response
Tiina Blomster1, Jarkko Salojärvi, Nina Sipari
1Division of Plant Biology, Department of Biosciences, University of Helsinki, FI-00014 Helsinki, Finland.
Plant Physiology
|October 19, 2011
Summary
Reactive oxygen species (ROS) transiently suppress auxin signaling in plants, a novel mechanism distinct from known pathways. Jasmonic acid (JA), not auxin, regulates ROS-induced cell death and stress-induced leaf morphology.
Area of Science:
- Plant biology
- Molecular plant science
- Plant stress physiology
Background:
- Reactive oxygen species (ROS) are crucial signaling molecules in plant development and stress responses.
- Understanding the plant's transcriptional response to apoplastic ROS is vital for deciphering stress signaling pathways.
Purpose of the Study:
- To investigate the plant transcriptional response to apoplastic ROS using ozone as a model inducer in Arabidopsis.
- To elucidate the interaction between ROS signaling and plant hormone pathways, particularly auxin.
Main Methods:
- Gene expression analysis using microarrays in Arabidopsis thaliana.
- Utilizing a DR5-uidA auxin reporter construct to monitor auxin signaling.
- Employing mutants defective in auxin and jasmonic acid (JA) responses to study ROS-induced phenomena.
Main Results:
- Apoplastic ROS treatment transiently suppressed auxin signaling, contrasting with increased signaling of other stress hormones.
- Transcriptomic data revealed modifications in auxin homeostasis and signaling, including reduced expression of auxin receptors and Aux/IAA repressors.
- ROS-induced cell death was regulated by JA, not auxin, and chronic ROS exposure led to altered leaf morphology (stress-induced morphogenic response).
Conclusions:
- Apoplastic ROS regulate auxin responses through a novel mechanism, distinct from known biotic stress pathways.
- Jasmonic acid plays a key role in mediating ROS-induced cell death and stress-induced morphogenic responses.
- Auxin acts as a negative regulator of stress-induced morphogenic response in Arabidopsis rosettes.
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