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SlWRKY6 Mediates H2S Signaling and SlGRF1-SlGIF2 Module to Coordinately Regulate Plant Growth, Fruit yield and
Min Zhang1, Lisheng Guan1, Hongye Sun2
1School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
Plant Physiology
|July 15, 2026
Summary
Hydrogen sulfide (H2S) delays tomato fruit ripening and leaf senescence. A newly discovered module involving SlWRKY6, SlGRF1, and SlGIF2 integrates H2S signals to coordinate plant growth, yield, and ripening.
Area of Science:
- Plant Biology
- Molecular Biology
- Biochemistry
Background:
- Hydrogen sulfide (H2S) is a gaseous signaling molecule that influences plant senescence and fruit ripening through protein persulfidation.
- The precise molecular mechanisms by which H2S integrates fruit ripening with overall plant growth and development are not fully understood.
Purpose of the Study:
- To elucidate the molecular mechanism by which H2S coordinates tomato fruit ripening with plant growth and development.
- To identify key regulatory components involved in H2S-mediated signaling.
Main Methods:
- Analysis of H2S-defective mutants (sllcd1) and overexpression lines (SlWRKY6).
- Molecular assays including gene expression analysis, promoter binding assays, and protein interaction studies.
- Gene editing in SlGRF1 mutants and functional validation of target genes (SlCYCD3;1, SlCBSX5).
Main Results:
- H2S delays tomato fruit ripening and leaf senescence; H2S deficiency leads to premature ripening and stunted growth.
- SlWRKY6, targeted by H2S via persulfidation, represses SlGIF2 transcription; H2S alleviates this repression.
- The SlGRF1-SlGIF2 complex positively regulates plant growth and fruit yield while delaying ripening by activating SlCYCD3;1 and SlCBSX5.
Conclusions:
- A novel regulatory module (SlWRKY6-SlGRF1-SlGIF2) integrates H2S signaling to coordinate plant growth, fruit yield, and fruit ripening in tomato.
- This module provides a mechanistic link between H2S, cell cycle regulation (SlCYCD3;1), and H2S biosynthesis (SlCBSX5).
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