Patterns of Soil Microbial Diversity, Assembly, and Co-Occurrence Along a Natural Salinity Gradient in an Inland...

Natural inland saline-alkaline wetlands offer opportunities for evaluating microbial responses to long-term salinity stress. This study examined surface soils from non-saline, moderately saline, and hypersaline sites in the Luyang Lake wetland, measuring comprehensive edaphic variables (including SAR, ESP, carbonate/bicarbonate chemistry, moisture, DOC, and inorganic N) alongside bacterial and fungal communities via 16S rRNA and ITS sequencing. A coupled salinity-ion and nutrient gradient was identified, with hypersaline soils characterized by high Na⁺, Cl^-, SAR, and ESP alongside depleted organic carbon and nitrogen. Bacterial α-diversity exhibited a significant unimodal response along the salinity gradient (quadratic regression: p < 0.001), peaking at moderate salinity. Fungal Shannon diversity declined with increasing salinity, but fungal Chao1 richness showed a U-shaped response, highlighting domain-specific and metric-dependent patterns along the gradient. Community assembly analyses revealed contrasting dynamics: deterministic processes were more prevalent in bacterial assembly in hypersaline soils, while fungal assembly remained predominantly stochastic. Co-occurrence networks showed sparser topological structure in high-salinity soils. These patterns are consistent with domain-specific microbial variation along the gradient to coupled edaphic stressors in inland saline-alkaline wetlands.