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Updated: May 28, 2026

A 3-D Visualization Technique for Bone Remodeling in a Suture Expansion Mouse Model
Published on: August 18, 2023
Periostin-like-factor-induced bone formation within orthopedic maxillary expansion
1Department of Orthodontics, Institute of Stomatology, Nanjing Medical University, Nanjing, China.
Periostin-like factor (PLF) significantly accelerates bone mineral formation in expanded palatal sutures. This study shows PLF enhances bone growth in palatal suture expansion models.
Area of Science:
- Craniofacial development and bone biology.
Background:
- Palatal suture expansion is a common orthodontic procedure.
- Understanding molecular mechanisms that regulate bone formation in sutures is crucial for optimizing treatment outcomes.
Purpose of the Study:
- To investigate the effect of periostin-like factor (PLF) on bone mineral formation within an experimentally expanded palatal suture.
Main Methods:
- Organ culture of expanded palates from male C57BL/6 mice.
- Stimulation with PLF or BMP-7 for 8 days.
- Assessment of bone mineral formation using Raman microspectroscopy and histological examination.
Main Results:
- PLF and BMP-7 significantly increased bone mineral concentration at the osteogenic fronts of the palatal suture compared to unstimulated controls.
- Alkaline phosphatase activity and osteocalcin protein levels were significantly elevated in PLF- and BMP-7-treated groups.
Conclusions:
- Periostin-like factor (PLF) effectively promotes bone mineral formation in expanded palatal sutures.
- PLF demonstrates potential as a therapeutic agent to enhance bone regeneration in craniofacial applications.
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