Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 28, 2026

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

Published on: February 16, 2017

Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord.

Owen J Tamplin¹, Brian J Cox, Janet Rossant

¹Program in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada. otamplin@enders.tch.harvard.edu

Developmental Biology

|October 20, 2011

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Human stem cell-based embryo models: innovation, ethics, and policy.

Human reproduction (Oxford, England)·2026

Same author

Enhancing rigor and reproducibility: Implementing STAR Methods and the ISSCR Standards Checklist at Stem Cell Reports.

Stem cell reports·2026

Same author

GABA produced by multiple bone marrow cell types regulates hematopoietic stem and progenitor cells.

Stem cell reports·2025

Same author

Vcam1 in endothelial and stromal cells regulates hematopoietic stem cell contact with the niche.

bioRxiv : the preprint server for biology·2025

Same author

A chromosome-scale genome assembly and annotation of the tetraploid herb "epazote" (Dysphania ambrosioides).

G3 (Bethesda, Md.)·2025

Same author

GABA produced by multiple bone marrow cell types regulates hematopoietic stem and progenitor cells.

bioRxiv : the preprint server for biology·2025

Foxa2 is crucial for vertebrate body plan development. This study identifies novel Foxa2 target genes and enhancers in the notochord, advancing our understanding of embryonic patterning.

Area of Science:

Developmental Biology
Genetics
Molecular Biology

Background:

The node and notochord are vital for vertebrate body plan patterning.
Foxa2 is a critical transcription factor regulating notochord development.
Identifying Foxa2 targets is key to understanding notochord formation and function.

Purpose of the Study:

To identify novel notochord-enriched genes and potential Foxa2 target genes.
To discover and validate functional Foxa2-dependent enhancers in the notochord.
To build a comprehensive resource of notochord-expressed genes.

Main Methods:

Microarray-based gene expression screening of sorted embryonic notochord cells.
Whole mount in situ hybridization for validating gene expression specificity.

More Related Videos

Functional Cloning Using a Xenopus Oocyte Expression System

Functional Cloning Using a Xenopus Oocyte Expression System

Published on: January 30, 2016

Related Experiment Videos

Last Updated: May 28, 2026

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

Published on: February 16, 2017

Functional Cloning Using a Xenopus Oocyte Expression System

Functional Cloning Using a Xenopus Oocyte Expression System

Published on: January 30, 2016

Analysis of existing Foxa2 ChIP-seq data combined with zebrafish reporter assays to identify enhancers.

Main Results:

Identified a large resource of notochord-enriched genes, serving as candidate Foxa2 targets.
Discovered 7 novel notochord enhancers, many not predictable by sequence conservation.
Confirmed Foxa2 dependence of novel enhancers through mutation analysis.

Conclusions:

This study provides a valuable resource for notochord gene regulatory network research.
Novel Foxa2-dependent enhancers were identified, crucial for notochord development.
Integrated gene expression profiling and ChIP analysis effectively reveals developmental regulatory elements.