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Vanadium: genetical and biochemical investigations
G Bronzetti1, E Morichetti, C Della Croce
1Istituto di Mutagenesi e Differenziamento, CNR, Pisa, Italy.
Mutagenesis
|May 1, 1990
Summary
Ammonium metavanadate induced genetic mutations in yeast, with higher activity observed without metabolic activation. This compound also reduced cytochrome P450 levels and liver enzyme activity in mice.
Area of Science:
- Biochemistry
- Genetics
- Toxicology
Background:
- Ammonium metavanadate is a chemical compound with potential biological activity.
- Vanadium compounds are known to interact with biological systems, including enzymes and DNA.
Purpose of the Study:
- To investigate the genotoxic potential of ammonium metavanadate in yeast.
- To examine the effect of ammonium metavanadate on the hepatic monooxygenase system in mice.
Main Methods:
- Assessing mitotic gene conversion and point mutation frequencies in Saccharomyces cerevisiae D7 strain.
- Measuring cytochrome P450 levels and monooxygenase activities (aminopyrine N-demethylase, p-nitroanisole O-demethylase, 7-ethoxycoumarin O-deethylase) in mouse liver microsomes.
Main Results:
- Ammonium metavanadate increased gene conversion and revertant frequencies in yeast, with peak activity observed without metabolic activation.
- The compound likely undergoes biotransformation to vanadyl by yeast cells and hepatic S9 fraction.
- Ammonium metavanadate decreased cytochrome P450 levels and suppressed hepatic monooxygenase activity in mice.
Conclusions:
- Ammonium metavanadate exhibits genotoxic effects in yeast.
- The compound interferes with the hepatic monooxygenase system in mice, reducing enzyme activity and cytochrome P450 levels.