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Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence
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Alkaline phosphatase enzymatic signal amplification for fast, sensitive impedimetric DNA detection

Miriam Kaatz1, Holger Schulze, Ilenia Ciani

  • 1University of Edinburgh, College of Medicine and Veterinary Medicine, Division of Pathway Medicine, 49 Little France Crescent, Edinburgh, UK.

The Analyst
|October 20, 2011
PubMed

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...