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Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
Published on: May 12, 2023
The draft genome and transcriptome of Cannabis sativa
Harm van Bakel1, Jake M Stout, Atina G Cote
1Banting and Best Department of Medical Research and Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, M5S 3E1, Canada.
Genome Biology
|October 22, 2011
Summary
Researchers sequenced the Cannabis sativa genome, revealing genetic differences between marijuana and hemp. This discovery aids in developing therapeutic cannabis strains and improving hemp cultivation.
Area of Science:
- Genomics
- Plant Molecular Biology
- Cannabinoid Research
Background:
- Cannabis sativa has historical uses for fiber, food, medicine, and intoxication.
- Selective breeding created distinct marijuana and hemp varieties.
- The molecular basis of cannabinoid production remains largely unknown.
Purpose of the Study:
- To sequence the genome and transcriptome of Cannabis sativa.
- To compare gene expression between marijuana and hemp.
- To identify genetic factors influencing cannabinoid profiles.
Main Methods:
- Whole-genome and transcriptome sequencing of marijuana (Purple Kush) and hemp ('Finola', 'USO-31').
- Comparative analysis of gene expression and copy number variations.
- Single nucleotide variant analysis.
Main Results:
- A draft haploid genome (534 Mb) and transcriptome (30,000 genes) for Purple Kush were generated.
- Marijuana showed higher expression of cannabinoid synthesis genes compared to hemp.
- Differences in key enzymes (THC vs. CBD synthase) and genetic variations distinguish marijuana from hemp.
Conclusions:
- The Cannabis sativa genome sequence facilitates research into its diverse applications.
- This resource supports the development of marijuana with specific cannabinoid profiles.
- It provides a foundation for breeding hemp with enhanced agricultural traits.
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