[Methods of in vitro evaluation for nephrotoxicity]

Related Experiment Video

Updated: Sep 7, 2026

Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury
07:58

Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury

Published on: July 17, 2016

N Castaing1, D Merlet, J Cambar

  • 1Groupe d'Etude de Physiologie et Physiopathologie Rénales, Faculté de Pharmacie, Bordeaux.

Journal De Toxicologie Clinique Et Experimentale
|March 1, 1990
PubMed

Insights

This review explores advanced in vitro models for assessing kidney toxicity, overcoming challenges posed by renal tissue complexity. These models aid in identifying molecular targets of nephrotoxicants more precisely.

Area of Science:

  • * Renal toxicology and molecular pharmacology.
  • * In vitro model development and validation.

Context:

  • * Traditional animal models for nephrotoxicant studies face limitations due to renal tissue heterogeneity.
  • * Identifying precise molecular targets of kidney toxicants at the cellular level remains challenging.
  • * Advancements in in vitro techniques offer more reductionist approaches.

Purpose:

  • * To review and present recent, increasingly reductionist in vitro models for assessing nephrotoxicity.
  • * To cover models including isolated kidneys, kidney slices, isolated renal cells, and cultured renal cells.
  • * To evaluate the utility of these models in identifying molecular targets of novel molecules.

Summary:

  • * Discusses the historical development and principles of various in vitro renal models.
  • * Details viability criteria and the specific advantages of each model for renal toxicology.
  • * Presents a comparative analysis of isolated kidneys, kidney slices, isolated cells, and cell cultures.
  • * Highlights the relative merits and limitations of each model in assessing molecule-induced kidney damage.

Impact:

  • * Provides a comprehensive overview of current in vitro methodologies for nephrotoxicity assessment.
  • * Facilitates the selection of appropriate models for evaluating the renal safety of new chemical entities.
  • * Contributes to a better understanding of molecular mechanisms underlying drug-induced kidney injury.

More Related Videos

Assessment of Kidney Function in Mouse Models of Glomerular Disease09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure08:25

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Published on: June 5, 2020

Related Concept Videos

In Vitro Drug Dissolution: Compendial Testing Models II01:09

In Vitro Drug Dissolution: Compendial Testing Models II

Various dissolution methods are utilized to assess a drug’s dissolution rate, including the flow-through cell, paddle-over-disk, cylinder, and reciprocating disk methods.The flow-through cell apparatus (USP (United States Pharmacopeia) method 4) comprises a reservoir for the dissolution medium and a pump that propels the medium through the cell containing the test sample. This method is crucial for assessing modified-release dosage forms with minimally soluble active ingredients, maintaining...
In Vitro Drug Dissolution: Alternative Methods01:17

In Vitro Drug Dissolution: Alternative Methods

Alternative drug dissolution methods include the rotating bottle, intrinsic dissolution test, peristalsis, and the Franz diffusion cell method. The rotating bottle method involves meticulously rotating tightly capped controlled-release beads in a temperature-controlled bath. Periodic decanting of samples allows for residue assay, followed by refilling with fresh medium and testing at various pH levels to emulate the gastrointestinal tract conditions.In contrast, the intrinsic dissolution test...
Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate01:25

Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate

The glomerular filtration rate (GFR) is a critical indicator of kidney health, reflecting how well the kidneys filter blood. Changes in GFR can signal potential kidney impairment, necessitating accurate measurement methods to monitor kidney function effectively.Various molecules can serve as markers for GFR measurement, with the ideal marker meeting several specific criteria. It must freely filter at the glomerulus, avoid reabsorption or secretion by the renal tubules, remain unmetabolized, not...
Toxicity Testing in Animals01:23

Toxicity Testing in Animals

Toxicity tests in animals are grounded on two main assumptions: first, the effects observed in laboratory animals can be extrapolated to humans, especially when adjusted for body surface area; second, high-dose exposure in animals is essential to identify potential human hazards from lower doses. This is based on the quantal dose-response concept, which faces the challenge of extrapolating results from relatively few test animals to much larger human populations. For example, a 0.01% incidence...