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Related Concept Videos

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

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Related Experiment Video

Updated: May 28, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Aptamer-functionalized microgel particles for protein detection.

Rathi L Srinivas1, Stephen C Chapin, Patrick S Doyle

  • 1Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Analytical Chemistry
|October 25, 2011
PubMed
Summary
This summary is machine-generated.

This study presents a new method for highly sensitive protein detection using aptamer-functionalized hydrogel microparticles. This advancement enables precise analysis of clinically relevant proteins in complex biological samples.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Clinical proteomics requires sensitive and multiplexed detection of proteins in complex biological samples.
  • Aptamers offer specificity and affinity for protein targets, suitable for particle-based detection systems.

Purpose of the Study:

  • To demonstrate highly sensitive detection of human α-thrombin using aptamer-functionalized hydrogel microparticles.
  • To evaluate the microgels' detection capabilities in sandwich-assay formats for clinical proteomics applications.

Main Methods:

  • Functionalization of encoded hydrogel microparticles with aptamer capture sequences.
  • Utilizing static imaging and microfluidic flow-through analysis for detection.
  • Employing sandwich-assay formats with aptamers and antibodies for target reporting.

Main Results:

  • Achieved highly sensitive detection of human α-thrombin with a limit of detection of 4 pM.
  • Demonstrated a three-log dynamic range using a three-dimensional, nonfouling hydrogel scaffold.
  • Optimized buffers and reagent concentrations for maximum reaction efficiency and a simple workflow.

Conclusions:

  • Aptamer-functionalized hydrogel microparticles enable sensitive and specific protein detection.
  • The developed assay is adaptable for multiplexed detection of other clinically relevant proteins.
  • This method advances clinical proteomics by providing a simple yet powerful detection tool.