Aptamer biosensor for label-free square-wave voltammetry detection of angiogenin

Area of Science:

• Biomedical Engineering
• Electrochemistry
• Biosensing

Background:

• Angiogenin (Ang) is a potent angiogenic factor implicated in tumor growth and metastasis.
• Accurate detection of Ang is crucial for cancer diagnosis and monitoring.
• Existing detection methods can be complex or lack sensitivity.

Purpose of the Study:

• To develop a simple, label-free square-wave voltammetry (SWV) aptasensor for sensitive angiogenin detection.
• To establish a rapid and selective method for quantifying Ang in biological samples.
• To demonstrate a promising strategy for detecting other protein biomarkers.

Main Methods:

• Utilized an anti-angiogenin aptamer as the molecular recognition element on a gold electrode.
• Employed the ferro/ferricyanide redox couple as a probe for SWV measurements.
• Monitored the suppression of SWV current upon Ang binding to quantify the analyte.

Main Results:

• The aptasensor demonstrated a linear response for Ang detection over a concentration range of 0.01 nM to 30 nM.
• Achieved a low detection limit of 1 pM for angiogenin.
• Exhibited excellent selectivity for Ang, unaffected by other proteins present in serum.

Conclusions:

• The developed SWV aptasensor offers a rapid, selective, and sensitive method for angiogenin detection.
• This label-free approach represents a significant advancement for cancer biomarker detection.
• The strategy holds promise for the development of aptasensors for a wide range of protein targets.