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Preparation and Structural Evaluation of Epithelial Cell Monolayers in a Physiologically Sized Microfluidic Culture Device
Published on: July 1, 2022
Evaluation of vertical cell fluidity in a multilayered sheet of skeletal myoblasts
Masahiro Kino-oka1, Trung Xuan Ngo, Eiji Nagamori
1Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan. kino-oka@bio.eng.osaka-u.ac.jp
Journal of Bioscience and Bioengineering
|October 25, 2011
Abstract:
The procedure for fabricating a multilayered cell sheet has been developed by combining multiple sheets using a thermo-responsive surface and stamp system. Confocal laser scanning microscopy revealed that the fluidity of a multilayered sheet of skeletal myoblasts could be estimated as vertical diffusivity and changed upon addition of dermal fibroblasts.
