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Pro-MMP-9/TIMP-1 ratio correlates poorly with a direct assessment of MMP-9 activity
G P Tarr1, M J A Williams, L V Phillips
1Department of Surgical Sciences, University of Otago, Dunedin, New Zealand.
Objective:
To determine if the pro-MMP-9/TIMP-1 ratio is an accurate surrogate for endogenously active MMP-9 levels.
Methods:
Plasma active MMP-9, pro-MMP-9 and TIMP-1 were measured in 295 patients.
Results:
There was a weak negative correlation between the pro-MMP-9/TIMP-1 ratio and active MMP-9. TIMP-1 was more closely correlated with active MMP-9 than pro-MMP-9.
Conclusion:
Pro-MMP-9/TIMP-1 ratio measured with ELISA is not a good surrogate measure for active MMP-9, and direct measurements of active MMP-9 are therefore recommended.
