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Negative-strand RNA replication by Q beta and MS2 positive-strand RNA phage replicases
1Department of Biochemistry, Texas College of Osteopathic Medicine/University of North Texas, Fort Worth 76107.
Virology
|September 1, 1990
Abstract:
Plasmids were constructed which allowed preparation of positive- and negative-strand MS2 RNA by in vitro transcription and which expressed the MS2 phage replicase. Transcribed, positive-strand MS2 RNA was infectious to Escherichia coli spheroplasts. Transcribed, negative-strand MS2 RNA was infectious to spheroplasts containing either MS2 replicase or related bacteriophage Q beta replicase.