A novel enzymatic hydrogen peroxide biosensor based on Ag/C nanocables

Area of Science:

• Nanomaterials science
• Electrochemistry
• Biosensor technology

Background:

• Hydrogen peroxide (H2O2) is a key analyte in biological and environmental systems.
• Development of sensitive and stable electrochemical sensors is crucial for H2O2 detection.
• Nanocomposite materials offer unique properties for enhanced sensor performance.

Purpose of the Study:

• To fabricate a novel enzymatic hydrogen peroxide sensor.
• To investigate the electrochemical properties of Ag/C nanocable and gold nanoparticle (AuNP) nanocomposites.
• To evaluate the sensor's performance for H2O2 detection.

Main Methods:

• Synthesis of Ag/C nanocables via hydrothermal method.
• Assembly of AuNPs onto Ag/C nanocables.
• Characterization using XRD, TEM, SEM, and EDS.
• Electrochemical performance evaluation using cyclic voltammetry (CV) at a glassy carbon electrode (GCE).

Main Results:

• Successfully fabricated Ag/C/Au nanocomposites with good chemical stability and biocompatibility.
• Demonstrated excellent electrocatalytic activity for H2O2 reduction.
• Achieved a linear detection range of 6.7×10⁻⁹ to 8.0×10⁻⁶ M.
• Obtained a low detection limit of 2.2×10⁻⁹ M.
• Determined an apparent Michaelis-Menten constant of 51.7×10⁻⁶ M.

Conclusions:

• Ag/C/Au nanocomposites show significant potential for constructing highly sensitive electrochemical biosensors.
• The developed sensor offers a promising platform for H2O2 monitoring.
• The synergistic effect of Ag/C nanocables and AuNPs enhances sensor performance.