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Point-of-Care Meat Freshness Evaluation: Myoglobin-Involved Redox Reaction Analysis via Smartphone Imaging.

Hui Zhu1, Yue Jiang1, Yu-Ping Wei1

  • 1Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, School of Chemistry & Chemical Engineering, Anhui University, Hefei 230601, PR China.

Analytical Chemistry
|September 14, 2025
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Summary

A new smartphone platform uses the resazurin indicator to visually assess meat freshness. This low-cost, rapid test helps reduce food waste and protect consumer health by detecting spoilage indicators.

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Area of Science:

  • Food Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • Meat freshness is critical for food safety and public health.
  • Current sensory methods for assessing meat quality are unreliable and lead to significant food waste.
  • Objective, rapid, and accessible methods for meat freshness evaluation are needed.

Purpose of the Study:

  • To develop a smartphone-based platform for visualizing meat freshness using a biocompatible redox indicator.
  • To establish a rapid, low-cost, and user-friendly method for home-based meat freshness testing.

Main Methods:

  • Utilized the redox indicator resazurin and smartphone imaging for colorimetric analysis.
  • Investigated the reducing activity of myoglobin (Fe2+) in fresh meat.
  • Developed algorithms to analyze color changes and generate heatmaps from RGB data.

Main Results:

  • Demonstrated a correlation between myoglobin Fe2+ content, reducing capacity, and meat freshness.
  • Successfully visualized distinct color transitions (blue to pink) of resazurin in response to meat freshness.
  • Validated the platform's effectiveness for pork, beef, and chicken within 30 minutes.

Conclusions:

  • The developed smartphone platform offers a rapid, low-cost, and non-toxic method for assessing meat freshness.
  • This technology has the potential to significantly reduce food waste and enhance consumer health protection.
  • The user-friendly nature promotes widespread adoption for home-based food safety monitoring.