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Related Experiment Video

Updated: May 28, 2026

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
09:51

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Parallel single-cell analysis microfluidic platform.

Floris T G van den Brink1, Elmar Gool, Jean-Philippe Frimat

  • 1BIOS-Lab on a Chip group, MESA+ Institute for Nanotechnology, University of Twente, The Netherlands.

Electrophoresis
|October 26, 2011
PubMed
Summary
This summary is machine-generated.

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This study introduces a polydimethylsiloxane (PDMS) microfluidic platform for parallel single-cell analysis (PaSCAl), enabling efficient cell trapping and in situ analysis to reveal cell population heterogeneity.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Cell population heterogeneity presents challenges in biological research.
  • Microfluidic platforms offer potential for precise single-cell analysis.

Purpose of the Study:

  • To develop and validate a polydimethylsiloxane (PDMS) microfluidic platform for parallel single-cell analysis (PaSCAl).
  • To demonstrate the platform's capability in trapping, staining, permeabilization, and content extraction from individual cells.
  • To analyze cell population heterogeneity using distinct cell lines.

Main Methods:

  • Single-cell trapping in PDMS microfluidic pockets with high capture yield (85%).
  • In situ staining of mixed cell populations using nucleic acid and antibody probes.

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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Related Experiment Videos

Last Updated: May 28, 2026

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
09:51

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

  • Chemical poration using digitonin or lithium dodecyl sulfate, visualized with fluorescent dyes.
  • Electrical protocol for cell permeabilization and content extraction via electroosmotic flow (EOF).

    • Main Results:

    • Achieved fast (2-5 min) and reproducible single-cell trapping.
    • Successfully differentiated cell types within a mixed population via in situ staining.
    • Demonstrated reversible and irreversible cell poration.
    • Validated electrical content extraction with successful recovery of intracellular contents.

    Conclusions:

    • The PaSCAl platform is a scalable and automatable tool for deciphering cell heterogeneity.
    • The platform enables diverse invasive and non-invasive single-cell analyses.
    • PaSCAl facilitates precise manipulation and analysis of individual cells within a population.