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Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
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[In vitro strategies for human gametes production from stem cells]

Lucie Tosca1, Anne-Marie Courtot, Annelise Bennaceur-Griscelli

  • 1Université Paris-Sud 11, hôpital Paul Brousse, Villejuif, France. lucie.tosca@abc.aphp.fr

Medecine Sciences : M/S
|October 27, 2011
PubMed

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Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.