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Selective two-photon fluorescence suppression by ultrafast pulse-pair excitation: control by selective one-color
Arijit Kumar De1, Debjit Roy, Debabrata Goswami
1Indian Institute of Technology Kanpur, Department of Chemistry, UP-208016, India.
Abstract:
Controlling two-photon molecular fluorescence leading to selective fluorophore excitation has been a long sought after goal in fluorescence microscopy. In this letter, we thoroughly explore selective fluorescence suppression through simultaneous two-photon absorption by two different fluorophores followed by selective one-photon stimulated emission for one particular fluorophore. We achieve this by precisely controlling the time delay between two identical ultrafast near infrared laser pulses.
