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R plasmids from clinical and environmental Klebsiella pneumoniae
1Lady Hardinge Medical College, New Delhi.
Abstract:
A total of 200 strains of K. pneumoniae, 150 from clinical and 50 from hospital environmental source were studied for antibiotic resistance pattern. The strains from clinical source showed higher incidence of drug resistance as compared to environmental strains. 50 clinical and 32 environmental multidrug resistant strains were tested for R plasmids. The biogroup 1 showed higher incidence of autotransferring and non autotransferring R plasmids.
Insights
Clinical Klebsiella pneumoniae strains exhibit greater antibiotic resistance than environmental ones. Multidrug-resistant strains frequently carry R plasmids, particularly in biogroup 1, impacting treatment strategies.
Area of Science:
- Microbiology
- Infectious Diseases
- Antimicrobial Resistance
Background:
- Klebsiella pneumoniae is a significant opportunistic pathogen.
- Antibiotic resistance in K. pneumoniae poses a global health threat.
- Understanding resistance patterns in clinical vs. environmental isolates is crucial.
Purpose of the Study:
- To investigate the antibiotic resistance patterns of K. pneumoniae from clinical and hospital environmental sources.
- To determine the prevalence and characteristics of R plasmids in multidrug-resistant K. pneumoniae strains.
Main Methods:
- Phenotypic antibiotic susceptibility testing was performed on 200 K. pneumoniae isolates (150 clinical, 50 environmental).
- Multidrug-resistant isolates were identified.
- R plasmid analysis was conducted on selected multidrug-resistant strains.
Main Results:
- Clinical K. pneumoniae isolates demonstrated a higher incidence of drug resistance compared to environmental isolates.
- Fifty clinical and 32 environmental multidrug-resistant strains were identified.
- Biogroup 1 strains showed a higher prevalence of both autotransferring and non-autotransferring R plasmids.
Conclusions:
- Clinical K. pneumoniae isolates are a more significant reservoir of antibiotic resistance.
- R plasmids are prevalent in multidrug-resistant K. pneumoniae, with specific characteristics in biogroup 1.
- These findings highlight the need for targeted infection control measures in healthcare settings.