Related Experiment Video
Updated: May 28, 2026
09:35
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
Published on: April 1, 2017
Proteomic analysis of Col11a1-associated protein complexes
Raquel J Brown1, Christopher Mallory, Owen M McDougal
1Department of Biological Sciences, Biomolecular Research Center and Musculoskeletal Research Institute, Boise State University, Boise, ID 83725-1515, USA.
Proteomics
|November 1, 2011
Summary
Researchers identified proteins interacting with collagen α1(XI) in cartilage, revealing key molecules involved in skeletal development and joint function. This enhances understanding of cartilage structure and aids tissue regeneration.
Area of Science:
- Biochemistry
- Molecular Biology
- Biomaterials Science
Background:
- Cartilage is crucial for skeletal development and joint function.
- Collagen fibrils and extracellular matrix (ECM) molecules maintain cartilage integrity and regulate fibrillogenesis.
- Understanding these interactions is vital for cartilage repair and tissue engineering.
Purpose of the Study:
- To identify proteins interacting with the amino terminal domain of collagen α1(XI) on cartilage collagen fibrils.
- To elucidate the role of collagen XI in cartilage fibrillogenesis and tissue integrity.
- To provide insights for cartilage regeneration strategies.
Main Methods:
- Affinity chromatography was used to isolate interacting proteins.
- Liquid chromatography/tandem mass spectrometry (LC-MS/MS) identified protein sequences.
- Proteins were extracted from fetal bovine cartilage using high ionic strength buffer.
Main Results:
- Proteins interacting with collagen α1(XI) were identified, including proteoglycans, collagens, and matricellular molecules.
- These interactions occur directly or indirectly with the collagen α1(XI) amino terminal domain.
- Identified proteins are involved in fibrillogenesis and tissue integrity maintenance.
Conclusions:
- Characterization of these molecular interactions deepens the understanding of cartilage ECM assembly.
- Collagen XI plays a significant role in cartilage fibrillogenesis and maintaining tissue integrity.
- Findings support advancements in tissue engineering and cartilage regeneration for treating damage and degeneration.
More Related Videos
Related Concept Videos
02:26Protein Networks
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
01:57Protein Complexes with Interchangeable Parts
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
01:57Protein Complexes with Interchangeable Parts
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
02:41Protein Complex Assembly
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
02:41Protein Complex Assembly
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
01:33Proteomics
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...