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Published on: March 19, 2015
[Construction of a recombination plasmid labelled with green fluorescence and its application in Clonostachys rosea]
Qiuhong Niu1, Tao Ke, Lin Zhang
1School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China. qiuhongniu@yahoo.com.cn
Objective:
We constructed a recombinant eukaryotic expression vector harboring green fluorescent protein (GFP) and the hygromycin resistance gene hph, and observed its expression in Clonostachys rosea.
Methods:
We used PCR, enzyme digestion, phosphorylation, ligation and transformation to construct the plasmid. Using protoplast preparation and transformation technologies, we expressed the plasmid in the fungi C. rosea.
Results:
We created the eukaryotic expression vector, transformed it into C. rosea and observed green fluorescence with fluorescence microscope.
Conclusion:
The successful construction of the pANGH3 recombinant plasmid and its expression in C. rosea establishes a new model for studying fungal infection mechanisms.
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