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Updated: May 28, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
08:51

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Published on: November 4, 2016

[Use of real time PCR for testing Staphylococcus aureus isolates from the Iaşi Infectious Diseases Hospital]

Teodora Vremeră1, Luminiţa Smaranda Iancu, Cătălina Logigan

  • 1Universităţii de Medicină şi Farmacie "Gr. T. Popa" Iaşi.

Revista Medico-Chirurgicala a Societatii De Medici Si Naturalisti Din Iasi
|November 4, 2011
PubMed
Abstract

Insights

Real-Time PCR rapidly detects methicillin-resistant Staphylococcus aureus (MRSA) and Panton-Valentine leukocidin (PVL) genes. This method aids in monitoring MRSA and PVL-producing strains for better epidemiological surveillance.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Infectious Diseases

Background:

  • Staphylococcus aureus causes diverse diseases and rapidly develops antibiotic resistance.
  • Monitoring S. aureus for resistance genes and virulence factors like Panton-Valentine leukocidin (PVL) is crucial.
  • Early detection of methicillin resistance and PVL is essential for effective treatment and control.

Purpose of the Study:

  • To determine the presence of mecA and pvl genes in S. aureus isolates using Real-Time PCR (RT-PCR).
  • To shorten the detection time for methicillin resistance and PVL gene presence.
  • To validate RT-PCR as a rapid diagnostic tool for S. aureus.

Main Methods:

  • 119 S. aureus strains isolated from pus were analyzed.
  • Phenotypic methods were used for methicillin resistance characterization per CLSI guidelines.
  • Detection of mecA and pvl genes was performed using RT-PCR with hydrolysis probes.

Main Results:

  • The prevalence of methicillin-resistant S. aureus (MRSA) was 40.33%.
  • The pvl gene was detected in 52.08% of the analyzed S. aureus strains.
  • RT-PCR results validated conventional methods for methicillin resistance detection.

Conclusions:

  • RT-PCR is an effective tool for the rapid detection of mecA and pvl genes in S. aureus.
  • The technique is valuable for epidemiological surveillance of MRSA and PVL-producing strains.
  • RT-PCR validates phenotypic resistance test results for oxacillin.

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