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Updated: May 27, 2026

Multispectral Optoacoustic Tomography for Functional Imaging in Vascular Research
Published on: June 8, 2022
Multispectral optoacoustic tomography by means of normalized spectral ratio
Thomas Jetzfellner1, Amir Rosenthal, Andreas Buehler
1Institute for Biological and Medical Imaging, Technische Universität München and Helmholtz Zentrum München, Ingolstaedter Landstr. 1, 85764 Neuherberg, Germany.
Abstract:
Quantification of biomarkers using multispectral optoacoustic tomography can be challenging due to photon fluence variations with depth and spatially heterogeneous tissue optical properties. Herein we introduce a spectral ratio approach that accounts for photon fluence variations. The performance and imaging improvement achieved with the proposed method is showcased both numerically and experimentally in phantoms and mice.

