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Updated: May 27, 2026
09:11
Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)
Published on: June 14, 2012
[Progress in zinc finger nuclease engineering for targeted genome modification]
An Xiao1, Ying-Ying Hu, Wei-Ye Wang
1Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China. an.xiao@163.com
Yi Chuan = Hereditas
|November 5, 2011
Summary
Zinc finger nucleases (ZFNs) are engineered proteins enabling precise genome editing by creating targeted DNA breaks. This technology facilitates gene modification for various applications in different species.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Context:
- Zinc finger nuclease (ZFN) technology represents a significant advancement in genome engineering.
- ZFNs are hybrid proteins combining DNA-binding zinc finger domains with the Fok I endonuclease.
- They enable targeted DNA recognition and cleavage, facilitating precise genomic modifications.
Purpose:
- To review the recent progress and prospects of ZFN technology for targeted genome modifications.
- To detail the mechanism of ZFN action, including target assessment and ZFP library construction.
- To provide insights into ZFN-mediated gene targeting, addition, and correction strategies.
Summary:
- ZFNs are engineered nucleases that bind specific DNA sequences and induce double-strand breaks (DSBs).
- This capability allows for precise manipulation of genomes, including gene disruption, addition, and correction.
- The review covers ZFN mechanisms, screening methods, modification strategies, and successful applications across species.
Impact:
- ZFN technology offers broad applications for generating inheritable mutations at cellular and organismal levels.
- It serves as a powerful tool for researchers studying gene function and developing novel genetic therapies.
- This review provides a valuable reference for scientists utilizing ZFNs in diverse research areas.
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