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Related Experiment Video

Updated: May 27, 2026

The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

Published on: April 7, 2023

Generating intestinal tissue from stem cells: potential for research and therapy.

Jonathan C Howell¹, James M Wells

¹Division of Endocrinology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229-3039, USA.

Regenerative Medicine

|November 5, 2011

Summary

Human pluripotent stem cells can be directed to differentiate into intestinal tissue in vitro. This breakthrough offers a promising new source for intestinal tissue engineering, disease modeling, and potential therapeutic transplantation.

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Last Updated: May 27, 2026

The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

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Published on: April 7, 2023

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Published on: July 9, 2021

Innervation of Human Intestinal Organoids

Innervation of Human Intestinal Organoids

Published on: January 17, 2025

Area of Science:

Regenerative Medicine
Developmental Biology
Gastroenterology

Background:

Intestinal resection and malformations cause severe health issues in patients of all ages.
Allogeneic intestinal transplantation faces significant challenges and limited success compared to other organ transplants.
Current in vitro intestinal tissue engineering methods, like organoids and embryoid bodies, have limitations.

Purpose of the Study:

To investigate the potential of human pluripotent stem cells (hPSCs) for directed differentiation into intestinal tissue.
To explore the application of hPSCs in creating functional intestinal tissue for research and therapeutic purposes.

Main Methods:

Utilizing principles from developmental biology to guide hPSC differentiation.
Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro.

Main Results:

Successfully demonstrated the directed differentiation of hPSCs into intestinal tissue.
Established a novel method for generating intestinal tissue from pluripotent stem cells.

Conclusions:

Human pluripotent stem cells provide a viable and promising source for generating intestinal tissue.
This approach holds significant potential for advancing intestinal disease modeling and therapeutic transplantation strategies.