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Related Experiment Videos

Some newly characterized collagenases from procaryotes and lower eucaryotes.

B Keil

    Molecular and Cellular Biochemistry
    |January 26, 1979
    PubMed
    Summary

    This study reviews four collagenases from bacteria and lower eukaryotes, detailing their chemical and enzymatic properties. Bacterial collagenases are zinc metallo-enzymes, while eukaryotic ones are serine proteinases, with varying specificities for collagen degradation.

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    Area of Science:

    • Biochemistry
    • Enzymology
    • Microbiology

    Background:

    • Collagenases are crucial enzymes for collagen degradation.
    • Understanding diverse collagenase properties aids in biochemical and medical research.

    Purpose of the Study:

    • To review and compare the chemical and enzymatic properties of four newly isolated collagenases.
    • To discuss the biosynthesis and precursors of these enzymes.
    • To analyze their substrate specificity and mechanism of collagen cleavage.

    Main Methods:

    • Isolation and purification of collagenases from Clostridium histolyticum, Achromobacter iophagus, Entomophthora coronata, and Hypoderma lineatum.
    • Characterization of enzymatic properties, including specific activity and substrate specificity.
    • Analysis of molecular weight, subunit composition, and amino acid composition.

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  • Comparison with known collagenases and related proteases.

    • Main Results:

    • Bacterial collagenases (Clostridium, Achromobacter) are Zn-metallo-enzymes; eukaryotic collagenases (Entomophthora, Hypoderma) are serine proteinases.
    • Achromobacter collagenase exhibits the highest specific activity and unique autolytic properties.
    • Collagen cleavage by bacterial collagenases occurs in the central helical region, with specificity differing from vertebrate collagenases.
    • Hypoderma collagenase shows homology to the trypsin family.

    Conclusions:

    • Diverse collagenases possess distinct biochemical and enzymatic characteristics.
    • Bacterial and eukaryotic collagenases present unique mechanisms and specificities in collagen degradation.
    • Comparative analysis reveals evolutionary relationships and functional similarities/differences among collagenases.