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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...

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Related Experiment Video

Updated: May 27, 2026

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer
07:52

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer

Published on: April 12, 2017

Single nuclei Raman spectroscopy for drug evaluation.

Hsin-Hung Lin1, Yen-Chang Li, Chih-Hao Chang

  • 1Genomics Research Center, Academia Sinica, Taipei, Taiwan.

Analytical Chemistry
|November 8, 2011
PubMed
Summary

Single nuclei Raman spectroscopy detects cellular changes from antineoplastic drugs faster and more sensitively than traditional assays. This method offers rapid, label-free molecular imaging for biopharmaceutical applications.

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An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
07:37

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

Related Experiment Videos

Last Updated: May 27, 2026

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer
07:52

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer

Published on: April 12, 2017

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
07:37

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

Area of Science:

  • Biomedical research
  • Biopharmaceutical applications
  • Single-cell analysis

Background:

  • Single-cell detection is crucial for biomedical and biopharmaceutical applications.
  • Raman spectroscopy enables label-free, time-resolved molecular imaging within single cells.

Purpose of the Study:

  • To monitor cellular responses to antineoplastic drugs at the single-cell level using Raman spectroscopy.
  • To evaluate the efficacy of single nuclei Raman spectroscopy in detecting drug-induced cytotoxicity.

Main Methods:

  • Utilized Raman spectroscopy for molecular imaging of single cells.
  • Focused on analyzing the spectral changes within isolated cell nuclei.
  • Compared the sensitivity and speed of nuclei Raman spectroscopy to conventional cell-based assays.

Main Results:

  • Single nuclei Raman spectroscopy detected nuclear changes associated with cytotoxicity.
  • The method identified cellular responses to antineoplastic drugs at lower concentrations and shorter time spans.
  • Demonstrated superior sensitivity and speed compared to conventional assays.

Conclusions:

  • Single nuclei Raman spectroscopy is a valuable tool for rapid and sensitive detection of cellular changes.
  • This technique shows significant potential for evaluating drug responses in biopharmaceutical research.
  • The label-free, single-cell approach offers a powerful alternative for cytotoxicity assessment.