Differentiation of raw meat from phylogenically related species by enzyme-linked immunosorbent assay

R M Patterson1, T L Spencer

  • 1Victorian Department of Agriculture, Regional Veterinary Laboratory, PO Box 388, Benalla, Victoria 3672, Australia.

Meat Science
|November 8, 2011
PubMed

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.