TPS1 terminator increases mRNA and protein yield in a Saccharomyces cerevisiae expression system
Mamoru Yamanishi1, Satoshi Katahira, Takashi Matsuyama
1TOYOTA Central R&D Labs, Inc, Nagakute, Aichi, Japan.
Bioscience, Biotechnology, and Biochemistry
|November 8, 2011
Abstract:
Both terminators and promoters regulate gene expression. In Saccharomyces cerevisiae, the TPS1 terminator (TPS1t), coupled to a gene encoding a fluorescent protein, produced more transgenic mRNA and protein than did similar constructs containing other terminators, such as CYC1t, TDH3t, and PGK1t. This suggests that TPS1t can be used as a general terminator in the development of metabolically engineered yeast in high-yield systems.
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