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Updated: May 27, 2026

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
Published on: October 6, 2019
In silico feedback for in vivo regulation of a gene expression circuit
Andreas Milias-Argeitis1, Sean Summers, Jacob Stewart-Ornstein
1Department of Electrical Engineering, ETH Zurich, Automatic Control Laboratory, Zurich, Switzerland.
Abstract:
We show that difficulties in regulating cellular behavior with synthetic biological circuits may be circumvented using in silico feedback control. By tracking a circuit's output in Saccharomyces cerevisiae in real time, we precisely control its behavior using an in silico feedback algorithm to compute regulatory inputs implemented through a genetically encoded light-responsive module. Moving control functions outside the cell should enable more sophisticated manipulation of cellular processes whenever real-time measurements of cellular variables are possible.
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