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Related Concept Videos

Cell Culture01:21

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
Cell Lines01:16

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
Plant Tissue Culture02:57

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
What is the Cell Cycle?01:04

What is the Cell Cycle?

The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...

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Related Experiment Video

Updated: May 27, 2026

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory
13:39

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory

Published on: July 7, 2023

Introduction to cell culture.

Christina Philippeos1, Robin D Hughes, Anil Dhawan

  • 1Institute of Liver Studies, King's College Hospital, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|November 8, 2011
PubMed
Summary
This summary is machine-generated.

This guide covers essential human cell culture techniques, emphasizing biosafety and aseptic conditions for reliable research. It details laboratory requirements, basic handling, and common assays for cell viability and activity.

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Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research

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Last Updated: May 27, 2026

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory
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Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory

Published on: July 7, 2023

Trypsinizing and Subculturing Mammalian Cells
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Published on: June 12, 2008

Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research
08:05

Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research

Published on: June 10, 2016

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microbiology

Background:

  • Human cell culture is crucial for biological research but requires strict safety protocols.
  • Working with human tissues necessitates understanding potential pathogenicity and implementing biosafety measures.
  • Maintaining aseptic conditions is vital to prevent microbial contamination in cell cultures.

Purpose of the Study:

  • To provide a comprehensive overview of fundamental human cell culture techniques.
  • To outline the necessary requirements for establishing and operating a tissue culture laboratory.
  • To discuss essential methods for cell isolation, purification, culture, and preservation.

Main Methods:

  • Detailed description of laboratory equipment for cell isolation, purification, and culture.
  • Explanation of aseptic techniques to prevent microbial contamination.
  • Discussion of basic cell handling, including media selection, primary culture initiation, and cryopreservation.
  • Overview of common assays for assessing cell viability and activity.

Main Results:

  • Established protocols for successful human cell culture.
  • Identified critical equipment and environmental controls for tissue culture labs.
  • Demonstrated techniques for maintaining culture integrity and cell health.
  • Outlined methods for long-term cell storage and functional assessment.

Conclusions:

  • Adherence to biosafety and aseptic techniques is paramount for successful human cell culture.
  • Proper laboratory setup and equipment are essential for reliable cell culture work.
  • Basic cell handling, cryopreservation, and viability assays are key components of cell culture practice.