Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Soft Tissue Augmentation Around Dental Implants - a Systematic Review.

Maedica·2026
Same author

The effect of P2RX7 functional SNPs on osteoblast cell phenotype, function and signalling- roles in bone homeostasis and osteogenesis.

Purinergic signalling·2026
Same author

Specific knockout of kidney homogentisate 1,2-dioxygenase reveals that local metabolism of tyrosine and homogentisic acid is negligible in alkaptonuria.

Human molecular genetics·2026
Same author

De novo Generalized Pustular Psoriasis: A Tofacitinib-Induced Paradoxical Adverse Event.

Indian dermatology online journal·2026
Same author

Evaluation of Preference of Pain Scale in Children using Novel Animated Emoji Scale in Nepal: An Observational Study.

JNMA; journal of the Nepal Medical Association·2026
Same author

Multiple Supernumerary Teeth with Concomitant Mandibular Hypo- Hyperdontia: A Case Report.

JNMA; journal of the Nepal Medical Association·2026

Related Experiment Video

Updated: May 27, 2026

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells
10:52

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

Published on: March 22, 2024

Human osteoclast culture and phenotypic characterization.

Ankita Agrawal1, James A Gallagher, Alison Gartland

  • 1The Mellanby Centre for Bone Research, Academic Unit of Bone Biology, The University of Sheffield, Sheffield, UK.

Methods in Molecular Biology (Clifton, N.J.)
|November 8, 2011
PubMed
Summary

This study details methods for isolating and culturing human osteoclasts from peripheral blood. These techniques aid in studying bone resorption and developing treatments for bone diseases like osteoporosis.

More Related Videos

Osteoclast Derivation from Mouse Bone Marrow
06:17

Osteoclast Derivation from Mouse Bone Marrow

Published on: November 6, 2014

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
08:07

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice

Published on: January 6, 2018

Related Experiment Videos

Last Updated: May 27, 2026

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells
10:52

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

Published on: March 22, 2024

Osteoclast Derivation from Mouse Bone Marrow
06:17

Osteoclast Derivation from Mouse Bone Marrow

Published on: November 6, 2014

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
08:07

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice

Published on: January 6, 2018

Area of Science:

  • Biomedical Science
  • Cell Biology
  • Skeletal Biology

Background:

  • Bone remodeling involves coupled bone resorption and formation, crucial for skeletal health.
  • Imbalances in bone remodeling lead to diseases like osteoporosis, osteopetrosis, and pycnodysostosis.
  • Understanding osteoclast function is vital for developing effective bone disease therapies.

Purpose of the Study:

  • To provide a comprehensive guide for isolating and culturing primary human osteoclasts in vitro.
  • To detail methods for characterizing osteoclasts and quantifying bone resorption.
  • To establish a robust toolkit for studying human osteoclasts and advancing bone disease treatments.

Main Methods:

  • Isolation of primary osteoclasts from human peripheral blood.
  • In vitro culturing techniques for human osteoclasts.
  • Methodologies for osteoclast characterization and in vitro resorption assays.

Main Results:

  • Established protocols for obtaining and maintaining human osteoclasts in laboratory settings.
  • Validated methods for assessing osteoclast morphology, function, and resorptive activity.
  • Demonstrated the utility of these combined methods for skeletal research.

Conclusions:

  • The presented methods offer a powerful tool for in-depth study of human osteoclasts.
  • This research facilitates the investigation of regulatory systems in bone resorption.
  • The developed techniques are instrumental in the pursuit of novel therapeutic strategies for bone diseases.