Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inter-tissue relationships of gene expression in liver, muscle and adipose tissue of children with end-stage chronic liver disease.

Journal of pediatric gastroenterology and nutrition·2026
Same author

New histopathological terminology for well-differentiated hepatocellular lesions in unusual clinico-pathological scenarios: HCA-like and FNH-like.

JHEP reports : innovation in hepatology·2026
Same author

Cryopreservation of hepatocytes: Impact of long-term storage and organ donor factors on cell quality for clinical applications.

Cell transplantation·2026
Same author

High Frequency of <i>CTNNB1</i> Variants Associated with Benign and Malignant Liver Tumors in Patients with Congenital Porto-Systemic Shunts.

Liver cancer·2025
Same author

Liver transplantation for hepatocellular adenomas: UK NHSBT-liver advisory group guidelines for patient selection.

HPB : the official journal of the International Hepato Pancreato Biliary Association·2025
Same author

The safety and efficacy of ultrasound histotripsy and human pluripotent stem cell-derived hepatic spheroid implantation as a potential therapy for treatment of congenital metabolic liver disease: Assessment in an immunocompetent rodent model.

Cell transplantation·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 27, 2026

Laser Capture Microdissection of Mammalian Tissue
16:34

Laser Capture Microdissection of Mammalian Tissue

Published on: October 1, 2007

Laser microdissection microscopy: application to cell culture.

Ahlam Mustafa1, Cathy Cenayko, Ragai R Mitry

  • 1Institute of Liver Studies, King's College London School of Medicine, London, UK. ahlam.mustafa@kcl.ac.uk

Methods in Molecular Biology (Clifton, N.J.)
|November 8, 2011
PubMed
Summary
This summary is machine-generated.

Laser microdissection (LMD) microscopy precisely isolates cells for molecular analysis. This technique enables RNA, DNA, and protein extraction from minimal cell samples for various downstream applications.

More Related Videos

Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture
12:38

Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture

Published on: September 16, 2013

Laser Capture Microdissection - A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area
08:29

Laser Capture Microdissection - A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area

Published on: February 6, 2015

Related Experiment Videos

Last Updated: May 27, 2026

Laser Capture Microdissection of Mammalian Tissue
16:34

Laser Capture Microdissection of Mammalian Tissue

Published on: October 1, 2007

Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture
12:38

Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture

Published on: September 16, 2013

Laser Capture Microdissection - A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area
08:29

Laser Capture Microdissection - A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area

Published on: February 6, 2015

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Microscopy

Background:

  • Precise isolation of specific cell populations is crucial for targeted molecular profiling.
  • Traditional methods may struggle to isolate sufficient material from small or heterogeneous samples.

Purpose of the Study:

  • To highlight the capabilities of Laser Microdissection (LMD) microscopy.
  • To demonstrate the utility of LMD for downstream molecular analyses.

Main Methods:

  • Utilizing Laser Microdissection (LMD) microscopy to precisely cut and isolate cells or tissues.
  • Applying LMD to histological sections, cytology preparations, and live cells from tissue cultures.

Main Results:

  • Successful isolation of specific cell populations using LMD microscopy.
  • Feasibility of RNA, DNA, and protein isolation from small cell numbers obtained via LMD.

Conclusions:

  • Laser Microdissection (LMD) microscopy is an effective technique for isolating specific cells for molecular studies.
  • Isolated cells are suitable for various downstream applications including real-time PCR, ELISA, Western Blotting, and protein microarrays.