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Imaging and 3D Reconstruction of Cerebrovascular Structures in Embryonic Zebrafish
Published on: April 22, 2014
Coagulation factor III (tissue factor) is required for vascularization in zebrafish embryos.
1Key Laboratory of Molecular Medicine, Department of Biochemistry and Molecular Biology, Ministry of Education, Shanghai Medical College, Fudan University, Shanghai, PR China.
Genetics and Molecular Research : GMR
|November 8, 2011
Summary
Coagulation factor IIIb (f3b) is crucial for zebrafish embryonic development, particularly for brain vascular formation. Its absence leads to developmental defects and abnormal vascular patterning.
Area of Science:
- Developmental Biology
- Hemostasis and Thrombosis
- Zebrafish Models
Background:
- Tissue factor (TF) initiates the extrinsic coagulation pathway.
- TF plays a vital role in embryonic development, with murine TF gene knockout causing high lethality.
- Zebrafish TF gene has paralogs, including coagulation factor IIIb (f3b).
Purpose of the Study:
- To investigate the role of coagulation factor IIIb (f3b) in zebrafish embryonic development.
- To analyze the expression patterns of endogenous f3b during embryogenesis.
- To determine the consequences of f3b deficiency on embryonic morphology and vascular development.
Main Methods:
- Expression analysis of endogenous f3b in zebrafish embryos.
- Knockout of f3b using morpholino injection at the one-to-two cell stage.
- Morphological and vascular patterning assessment of f3b morphant embryos.
Main Results:
- Endogenous f3b expression was observed in pectoral fins and pharyngeal regions.
- f3b morphants exhibited brain ventricle edema and bleeding.
- Abnormal vascular patterning was a consistent finding in f3b morphants.
Conclusions:
- Coagulation factor IIIb (f3b) is essential for normal zebrafish embryonic development.
- f3b plays a critical role in brain vascular development.
- f3b is also required for the development of certain somatic vasculature during embryogenesis.
